Journal: Scientific Reports

Article Title: Analysis of the spatial-temporal distribution and functional morphology of telocytes in goat testes

doi: 10.1038/s41598-026-36639-3

Figure Lengend Snippet: DIF of CD34/α-SMA in 1-month-old goat testis. ( A-C ) DIF of CD34/α-SMA in 1-month-old goat testis; ( D-F ) DIF of CD34/α-SMA in blood vessels of 1-month-old goat testis; CD34 (red); α-SMA (green); DAPI (blue); CD34 + cells (white triangular arrows); ST. Seminiferous tubule; BV. Blood vessels. Scale bar = ( A-C ):10 μm; ( D-F ): 20 μm.

Article Snippet: The antibody pairs were as follows: CD34 (1:100, BA3414, Boster Biological Technology Co., Ltd)/α-SMA (1:100, BM0002, Boster Biological Technology Co., Ltd), CD34 (1:100)/Vimentin (1:100, BM0135, Boster Biological Technology Co., Ltd), α-SMA (1:100)/PDGFR-α (1:100, 3174 S, CellSignaling Technology).

Techniques:

Journal: Scientific Reports

Article Title: Analysis of the spatial-temporal distribution and functional morphology of telocytes in goat testes

doi: 10.1038/s41598-026-36639-3

Figure Lengend Snippet: DIF of CD34/α-SMA in 2-month-old goat testis. ( A-C ) DIF of CD34/α-SMA in 2-month-old goat testis; ( D-F ) DIF of CD34/α-SMA in blood vessels of 2-month-old goat testis; CD34 (red); α-SMA (green); DAPI (blue); CD34 + cells (white triangular arrows); ST. Seminiferous tubule; BV. Blood vessels. Scale bar = ( A-C ): 20 μm; ( D-F ): 10 μm.

Article Snippet: The antibody pairs were as follows: CD34 (1:100, BA3414, Boster Biological Technology Co., Ltd)/α-SMA (1:100, BM0002, Boster Biological Technology Co., Ltd), CD34 (1:100)/Vimentin (1:100, BM0135, Boster Biological Technology Co., Ltd), α-SMA (1:100)/PDGFR-α (1:100, 3174 S, CellSignaling Technology).

Techniques:

Journal: Scientific Reports

Article Title: Analysis of the spatial-temporal distribution and functional morphology of telocytes in goat testes

doi: 10.1038/s41598-026-36639-3

Figure Lengend Snippet: DIF of CD34/α-SMA in 12-month-old goat testis. ( A-I ) DIF of CD34/α-SMA in 12-month-old goat testis; ( J-L ) DIF of CD34/α-SMA in blood vessels of 12-month-old goat testis; CD34 (red); α-SMA (green); DAPI (blue); ST. Seminiferous tubule; BV. Blood vessels. Scale bar = ( A-F, J-L ): 10 μm; ( G-I ): 20 μm.

Article Snippet: The antibody pairs were as follows: CD34 (1:100, BA3414, Boster Biological Technology Co., Ltd)/α-SMA (1:100, BM0002, Boster Biological Technology Co., Ltd), CD34 (1:100)/Vimentin (1:100, BM0135, Boster Biological Technology Co., Ltd), α-SMA (1:100)/PDGFR-α (1:100, 3174 S, CellSignaling Technology).

Techniques:

Journal: Scientific Reports

Article Title: Analysis of the spatial-temporal distribution and functional morphology of telocytes in goat testes

doi: 10.1038/s41598-026-36639-3

Figure Lengend Snippet: DIF of CD34/ Vimentin in 1-month-old goat testis. ( A-C ) DIF of CD34/ Vimentin in 1-month-old goat testis. (1-a), (1-b), (1-c) are respectively enlarged images of (1) in ( A ), ( B ), ( C ). (2-a), (2-b) and (2-c) are the enlarged images of (2) in ( A ), ( B ) and ( C ) respectively. CD34 (red); α-SMA (green); DAPI (blue); TC (yellow, indicated by white triangular arrows); ST. Seminiferous tubule; SC. Sertoli cells. Scale bar = ( A, B, C ): 20 μm; (1 - a) - (1 - c), (2- a) - (2 - c): 10 μm.

Article Snippet: The antibody pairs were as follows: CD34 (1:100, BA3414, Boster Biological Technology Co., Ltd)/α-SMA (1:100, BM0002, Boster Biological Technology Co., Ltd), CD34 (1:100)/Vimentin (1:100, BM0135, Boster Biological Technology Co., Ltd), α-SMA (1:100)/PDGFR-α (1:100, 3174 S, CellSignaling Technology).

Techniques:

Journal: Scientific Reports

Article Title: Analysis of the spatial-temporal distribution and functional morphology of telocytes in goat testes

doi: 10.1038/s41598-026-36639-3

Figure Lengend Snippet: DIF of CD34/ Vimentin in 2-month-old goat testis. ( A-C ) DIF of CD34/ Vimentin in 2-month-old goat testis. (a-c) are enlarged views of the boxed areas in ( A-C ). CD34 (red); α-SMA (green); DAPI (blue); TC (yellow, indicated by white triangular arrows); ST. Seminiferous tubule. Scale bar = ( A-C ): 20 μm; (a-c): 10 μm.

Article Snippet: The antibody pairs were as follows: CD34 (1:100, BA3414, Boster Biological Technology Co., Ltd)/α-SMA (1:100, BM0002, Boster Biological Technology Co., Ltd), CD34 (1:100)/Vimentin (1:100, BM0135, Boster Biological Technology Co., Ltd), α-SMA (1:100)/PDGFR-α (1:100, 3174 S, CellSignaling Technology).

Techniques:

Journal: Scientific Reports

Article Title: Analysis of the spatial-temporal distribution and functional morphology of telocytes in goat testes

doi: 10.1038/s41598-026-36639-3

Figure Lengend Snippet: DIF of CD34/ Vimentin in 12-month-old goat testis. ( A-C ) DIF of CD34/ Vimentin in 12-month-old goat testis. CD34 (red); Vimentin (green); DAPI (blue); ST. Seminiferous tubule; S. sperm; PS. Primary spermatocyte; SC. Sertoli cells; RS. Round sperm cells; White triangular arrows. Peritubular myoid cell nucleus. Scale bar = ( A-C ): 10 μm.

Article Snippet: The antibody pairs were as follows: CD34 (1:100, BA3414, Boster Biological Technology Co., Ltd)/α-SMA (1:100, BM0002, Boster Biological Technology Co., Ltd), CD34 (1:100)/Vimentin (1:100, BM0135, Boster Biological Technology Co., Ltd), α-SMA (1:100)/PDGFR-α (1:100, 3174 S, CellSignaling Technology).

Techniques:

Journal: Scientific Reports

Article Title: Analysis of the spatial-temporal distribution and functional morphology of telocytes in goat testes

doi: 10.1038/s41598-026-36639-3

Figure Lengend Snippet: DIF of CD34/ Vimentin in 12-month-old goat testis. ( A-C ) DIF of CD34/ Vimentin in 12-month-old goat testis. (a-c) is an enlarged view of the white dotted box area of ( A-C ). CD34 (red); Vimentin (green); DAPI (blue); TC (yellow, indicated by white triangular arrows). Scale bar = ( A-C ), (a-c): 20 μm.

Article Snippet: The antibody pairs were as follows: CD34 (1:100, BA3414, Boster Biological Technology Co., Ltd)/α-SMA (1:100, BM0002, Boster Biological Technology Co., Ltd), CD34 (1:100)/Vimentin (1:100, BM0135, Boster Biological Technology Co., Ltd), α-SMA (1:100)/PDGFR-α (1:100, 3174 S, CellSignaling Technology).

Techniques:

Journal: Scientific Reports

Article Title: Analysis of the spatial-temporal distribution and functional morphology of telocytes in goat testes

doi: 10.1038/s41598-026-36639-3

Figure Lengend Snippet: DIF of CD34/ Vimentin in 12-month-old goat testis. ( A-C ) DIF of CD34/ Vimentin in 12-month-old goat testis. (1-a), (1-b), (1-c) are respectively enlarged images of (1) in ( A-C ). (2-a), (2-b), (2-c) are the enlarged images of (2) in ( A-C ). CD34 (red); Vimentin (green); DAPI (blue); TC (yellow); White triangular arrows. Peritubular myoid cell nucleus. Scale bar = ( A-C ): 50 μm; (1-a) - (1-c), (2-a) - (2-c): 10 μm.

Article Snippet: The antibody pairs were as follows: CD34 (1:100, BA3414, Boster Biological Technology Co., Ltd)/α-SMA (1:100, BM0002, Boster Biological Technology Co., Ltd), CD34 (1:100)/Vimentin (1:100, BM0135, Boster Biological Technology Co., Ltd), α-SMA (1:100)/PDGFR-α (1:100, 3174 S, CellSignaling Technology).

Techniques:

Journal: Scientific Reports

Article Title: Retroviral infection of human neurospheres and use of stem Cell EVs to repair cellular damage

doi: 10.1038/s41598-022-05848-x

Figure Lengend Snippet: HTLV-1 co-culture and expression of cellular markers in HIV-1 and HTLV-1 infected neurospheres. (a) Differentiated neurospheres were exposed to dual-tropic HIV-1 89.6 with or without cART (lamivudine, tenofovir disoproxil fumarate, emtricitabine, indinavir), or were co-cultured with irradiated HTLV-1 infected cells with or without IFN/AZT for a period of fourteen days. Western blot was performed to examine the relative expression of various CNS-relevant cellular markers including those associated with microglia-like cells, astrocytes, neurons (dopaminergic, GABAergic, glutamatergic), and NSCs. (b) Western blot was performed to examine the relative expression of Tax and p19 in neurospheres co-cultured with HTLV-1 infected cells with or without IFN/AZT treatment. (c) RT-qPCR was performed to quantify the copy numbers of tax , env , and hbz RNA in neurospheres co-cultured with HTLV-1 infected cells with or without IFN/AZT treatment. n = 3. ** p < 0.005, *** p < 0.0001.

Article Snippet: After approximately 48 to 72 h, the medium was removed from the wells and replaced with DMEM:F12 (ATCC 30-2006) + Dopaminergic Neuron Differentiation Kit (ATCC ACS-3004) to induce differentiation.

Techniques: Co-Culture Assay, Expressing, Infection, Cell Culture, Irradiation, Western Blot, Quantitative RT-PCR